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EPIC Nutrient Database (ENDB) for Nutritional Epidemiology

Three components are necessary to investigate the relation between nutrition and health:

    • measuring the intake of food
    • converting the intake of food measurement into the intake of nutrients (which is usually based on national nutritional databases)
    • statistical analysis of the foods and nutrient intake data

Food composition tables were studied from each of the EPIC countries (except Norway), and comparisons made on the basis of availability, definition, analytical methods, and mode of expression of the nutrients of interest. Most of the nutrients in the tables are analysed and expressed in a compatible way but for some nutrients common methods and definitions or modes of expression have not yet been agreed upon, so values are not comparable.

In the tables that were compared the nutrients can be separated into 3 groups:

    1. Those with comparable values, although the definition and analytical methods may be slightly different:
      nitrogen, lactose, alcohol, water, cholesterol, fat, fatty acids, retinol, vitamin D, tocopherols, tocotrienols, thiamin, riboflavin, vitamins B6 and B12, calcium, iron and potassium
    2. Those which are not readily comparable due to the calculation or mode of expression but which may be easily converted to a comparable mode of expression:
      protein, carbohydrates, starch, sugars, energy, carotenes, vitamins A and E
    3. Those which are not comparable between all the tables studied due to the methods or definition used:
      folate and fibre (more from table below)

This has important implications for EPIC and it is, therefore, necessary to develop a standardized nutrient database in order to manage the food composition of the 10 participating countries. Existing software is being adapted to standardize and evaluate the data from these countries according to common rules, enabling a single database with comparable nutrient values from these 10 countries to be compiled.

An example of 3. above, folate, is demonstrated in the following table (not available in the published paper):

Folates/Vitamin B9: availability, definition and methods used

Country

Availability/

term used

Definition given

Method used

       

DK 96

Folates

-

Microbiological assay (not yet validated)

GB

Folate

Total folate forms

Microbiological assay after deconjugation of the polyglutamyl forms: Phillips and Wright, 1983; Bell, 1974

F 95

Folate

-

Microbiological assay after deconjugation of the polyglutamyl forms

F fat

Free folic acid

Clear from the name

No method mentioned

F milk

Free folic acid

Clear from the name

No method mentioned

F fruit

Total folates

Clear from the name

No method mentioned

D 79

Folic acid

-

No method mentioned

D milk

Folic acid

-

No method mentioned

D 93

Folic acid

Sum of free and bound folic acid forms

No method mentioned

D SFK

Folic acid

Sum of free and bound folic acid forms

Identified in footnotes: Microbiological assay or HPLC: H.Müller, Z. Lebensm. Unters. Forsch 196 (1993) 137

D BLS

Free folic acid

Bound folic acid

Free folic acid eq.

Total vitamin B9 ( in free folic acid eq.) = free folic acid + 0.2 bound folic acid

No method mentioned

GK 92

-

 

-

IT 94

-

-

IT 98

Folic acid

Total folates

Microbiological assay / microbiological and enzymatic method

NL 96

Folic acid

(in the PC-version only)

-

Microbiological assay after deconjugation of the polyglutamyl forms (not yet validated)

E 88

-

E 90

Free folic acid

Total folic acid

Clear from the names

Microbiological assay before and after treatment with conjugase

E 92

Folic acid

-

No method mentioned

E 93

Folic acid

Total folates

No method mentioned

E 97

Folic acid

-

HPLC: Shearer, 1986

S 94

Folic acid

Free and bound forms

Before 1981: Microbiological assay

After 1981: Binding-protein assay with a radioactive substrate (not yet validated for foods)

S 96

Folate

Free and bound forms

Idem S 94


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